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Shazshura The Legacy ECM coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to exm the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in.
Electrofusion is carried out by applying both the AC and DC pulse waveforms. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids. The LEGACY ECM is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications.
Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. Utilizing the Legacy ECM for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device.
Supplier BTX show all products. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined eccm standard cuvettes, high throughput plates, specialty electrodes and other available accessories. For use in hybridoma production applications, the Legacy ECM generator, 3. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab.
The Legacy ECM is ecm of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Read tuning-car-manuals After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids. The Ecm Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for mm petri dishes.
Majual switch-over time from AC to DC allows for highly efficient fusion. If you continue we assume that you consent to receive all cookies on this website. Blastomeres and oocytes can also be aligned to mnual correct position for better fusion accuracy during nuclear transfer and embryo manipulation.
Electroporate adherent cells directly into the dish used for cell growth. Multi-purpose electro cell manipulation generator capable of producing a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Cell fusion Nuclear transfer Embryo manipulation Hybridoma production Mammalian cell transfection Plant protoplast fusion Stem cell production.
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